Amide-Amide and Amide-Water Hydrogen Bonds: Implications for Protein Folding and Stability
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چکیده
منابع مشابه
Amide-Amide and Amide-Water Hydrogen Bonds: Implications for Protein Folding and Stability.
Amide-amide hydrogen bonds have been implicated in directing protein folding and enhancing protein stability. Inversion transfer (13)C NMR spectroscopy and IR spectroscopy were used to compare the ability of various amide solvents and of water to alter the rate of the cis-trans isomerization of the prolyl peptide bond of Ac-Gly-[β,δ-(13)C]Pro-OMe and the amide I vibrational mode of [(13)C=O]Ac-...
متن کاملProbing the role of backbone hydrogen bonds in protein-peptide interactions by amide-to-ester mutations.
One of the most frequent protein-protein interaction modules in mammalian cells is the postsynaptic density 95/discs large/zonula occludens 1 (PDZ) domain, involved in scaffolding and signaling and emerging as an important drug target for several diseases. Like many other protein-protein interactions, those of the PDZ domain family involve formation of intermolecular hydrogen bonds: C-termini o...
متن کاملDependence of amide vibrations on hydrogen bonding.
The effect of hydrogen bonding on the amide group vibrational spectra has traditionally been rationalized by invoking a resonance model where hydrogen bonding impacts the amide functional group by stabilizing its [(-)O-C=NH (+)] structure over the [O=C-NH] structure. However, Triggs and Valentini's UV-Raman study of solvation and hydrogen bonding effects on epsilon-caprolactum, N, N-dimethylace...
متن کاملThe contribution of C alpha-H...O hydrogen bonds to membrane protein stability depends on the position of the amide.
Structural analyses of membrane proteins reveal a large number of C(alpha)-H...O contacts between transmembrane helices, presumed to be hydrogen bonds. Recent experiments produced conflicting results for the contribution of such hydrogen bonds to membrane protein stability. An FTIR study estimated an energy of -0.88 kcal/mol for the G79-C(alpha)-H...I76-O hydrogen bond in glycophorin A, whereas...
متن کاملHeats of hydrolysis of amide and peptide bonds.
The thermodynamics of amide and peptide bonds is of fundamental importance in the study of the synthesis and breakdown of proteins in biological systems. One of the necessary steps in acquiring significant thermodynamic data in this field is the determination of the changes in heat content accompanying the formation or rupture of such bonds in compounds of known structure. We have accordingly u...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 1994
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja00084a067